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16 using SDSPAGE and western blotting with a 1:5,000 dilution of rabbit anti-His6 antibody (Abcam) followed by a 1:5,000 dilution of anti-rabbit-HRP (Sigma-Aldrich Chemie GmbH)
Shake the bottle and hold it about 2030 cm from your face
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Free Radic Biol Medic 122:420 Ding L, Charles MT, Carissea O, Tsao R, Dube C, Khanizadeh S (2011) Changes in ascorbateglutathione pathway enzymes in response to Mycosphaerella fragariae infection in selected strawberry genotypes